This antibody binds specifically to the C-terminal region of human CCR11, detecting the non-phosphorylated isoform. It is optimized for Western blot analysis and supports immunohistochemistry in formalin-fixed, paraffin-embedded tissue sections.
Applications
WB
Dilutions
WB: 1,000, IHC: 1:100
Reactivity
Human
Immunogen
Synthetic peptide corresponding to the C-terminal of human CCR11. Immunogen range is 22-22 amino acids.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Dulbecco's PBS, pH 7.4, with 150 mM NaCl and 0.005% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot validation of Atypical Chemokine Receptor 4 (CCR11) in transfected HEK293 cells. Lysates from native HEK293 cells (MOCK) or cells stably expressing CCR11 were immunoblotted with anti-CCR11 antibody (A334495) at 1:1000.
Immunohistochemical detection of Atypical Chemokine Receptor 4 (CCR11) in human spleen. Spleen sections were dewaxed, microwaved in citric acid, and stained with anti-CCR11 antibody (A334495) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. CCR11 receptors were observed at the plasma membrane of nearly all spleen cells.
Localization of Atypical Chemokine Receptor 4 (CCR11) in human spleen by immunohistochemistry. Spleen sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR11 antibody (A334495) at 1:100, followed by biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed with 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. CCR11 receptors were detected at the plasma membrane of nearly all spleen cells.
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