This antibody recognizes an extracellular epitope of CD193, an approximately 41 kDa protein expressed above all in eosinophils and basophils.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
5E8
Isotype
IgG2b
Light Chains
kappa
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
41 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
C C CKR3, C-C chemokine receptor type 3, CD193, CMKBR3, Eosinophil eotaxin receptor
Separation of human basophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CCR3 with Anti-CCR3 Antibody [5E8] (FITC) (A122117), (4 µl reagent / 100 µl of peripheral whole blood).
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