Mouse monoclonal [CVS9] antibody to CD11a/CD18 (PE).
Specificity
This antibody recognises the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells.In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Horse
Immunogen
Equine leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CVS9
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Horse CD11a/CD18 antibody, clone CVS9 recognizes the equine homolog of the human CD11a/CD18 cell surface antigen, a hetrodimer expressed on all equine cells of haemopoietic origin. Equine CD11a/CD18 has higher expression on some subpopulations of T-cells.In addition to the CVS9 clone, other CVS clones recognising equine MHC and cell surface antigens are available from Bio-Rad.
Figure A. Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC) (A280169) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-MHC Class I Monomorphic Antibody [CVS22] (FITC) (A280169) and Anti-CD11a/CD18 Antibody [CVS9] (PE) (A280187). All experiments performed on red cell lysed horse blood gated on lymphocytes in the presence of 10% horse seum. Data acquired on the ZE5 Cell Analyzer.
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