This antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and does not recognize CD11b on non-activated cells.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 1-4 µg/ml
Reactivity
Human
Immunogen
Human ITGAM.
Host
Mouse
Clonality
Monoclonal
Clone ID
CBRM1/5
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human PMA stimulated peripheral blood mononuclear cell suspension, labeling CD11b with Anti-CD11b Antibody [CBRM1/5] (A86820), (concentration in sample 4 µg/ml) GAM APC.
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