Anti-CD11b Antibody [M1/70.15] (PE) (A280989)

Anti-CD11b Antibody [M1/70.15] (PE)

Rat monoclonal [M1/70.15] antibody to CD11b (PE).

This antibody recognises the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent.

Mouse, Human, Rabbit

T cell enriched splenocytes from B10 mice.

Rat

Monoclonal

M1/70.15

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests: Reconstitute with 0.25 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995). This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3A, Integrin alpha-M, ITGAM, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

This product is for research use only. It is not intended for diagnostic or therapeutic use.