Anti-CD11c Antibody [3A8] (A320263)

Anti-CD11c Antibody [3A8]

Mouse monoclonal [3A8] antibody to CD11c.

This antibody recognises CD11c also known as integrin alpha-X.Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC). Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells.

Flow Cytometry: 1:20 - 1:100, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Porcine

Porcine alveolar macrophages.

Mouse

Monoclonal

3A8

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD11c, clone 3A8 recognizes CD11c also known as integrin alpha-X.Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC).Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells (Deloizy et al. 2016).

CD11 antigen-like family member C, Integrin alpha-X, ITGAX, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95