Mouse monoclonal [BU15] antibody to CD11c (APC-Cyanine 7).
Specificity
This antibody reacts with an extracellular epitope of CD11c, a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Monkey
Immunogen
Dendritic cells of synovial fluid.
Host
Mouse
Clonality
Monoclonal
Clone ID
BU15
Isotype
IgG1
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD11 antigen-like family member C, Integrin alpha-X, ITGAX, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD11c Antibody [BU15] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human monocytes (red-filled) from CD11c negative lymphocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD11c Antibody [BU15] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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Proteins predicted to interact with CD11c
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
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