Anti-CD11d Antibody [CA11.8H2] (A279970)

Anti-CD11d Antibody [CA11.8H2]

Mouse monoclonal [CA11.8H2] antibody to CD11d.

This antibody recognises the canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp.Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages with macrophages demonstrating marked erythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ ?/d T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Canine

Affinity purified CD11 antigen from canine spleen.

Mouse

Monoclonal

CA11.8H2

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Dog CD11d antibody, clone CA11.8H2 recognizes the canine CD11d cell surface antigen, also known as integrin alpha d subunit. The alpha d integrin (CD11d) subunit is expressed as a heterodimer with beta 2 integrin (CD18). CD11d is expressed by a sub-population of macrophages, some CD8 positive T cells and by gamma/delta T cells in the splenic red pulp.Over expression of CD11d is noted in histiocytic disorders including hemophagocytic sarcoma, described as a proliferative disorder specifically of CD11+ macrophages (Moore et al. 2006) with macrophages demonstrating marked erythrocytic phagocytosis. Previously, canine histiocytic sarcoma were described as originating entirely from interstitial dendritic cells. Cells from animals with canine hepatosplenic T cell lymphoma (HTSL) strongly express CD11d on malignant cells. Dogs normally demonstrate strong positivity for CD11d on large granular lymphocytes, macrophages and T cells in the splenic red pulp. CD11+ ?/d T cells constitute ~30% red pulp T cells despite their rarity in peripheral blood, suggesting a splenic red pulp origin of HTSL (Fry et al. 2003).

ADB2, CD11 antigen-like family member D, Integrin alpha-D, ITGAD, Leukointegrin alpha D