Anti-CD11R1 Antibody [MIL4] (A281209)

Anti-CD11R1 Antibody [MIL4]

Mouse monoclonal [MIL4] antibody to CD11R1.

This antibody recognises the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens. Mouse anti Pig CD11R1, clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages. Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin ß2 (CD18) of ~95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells.

IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Porcine, Human, Guinea Pig

Porcine Lamina Propria Leucocytes.

Mouse

Monoclonal

MIL4

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD11R1, clone MIL4 recognizes the porcine cell surface antigen classified as CD11R1 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). Mouse anti Pig CD11R1, clone MIL4 stains porcine eosinophils, a subset of neutrophils and NK cells, it does not stain monocytes or macrophages (Haverson et al. 1994).Mouse anti Pig CD11R1, clone MIL4 immunoprecipitates a band corresponding to integrin ß2 (CD18) of ~95kDa, in common with all other anti CD11 antibodies tested at the workshop and also a band of ~165 kDa corresponding to CD11R1, in a manner identical to the cross reactive anti human CD11b clone, TMG6-5 from peripheral blood mononuclear cell lysates suggesting that porcine CD11R1 is analogous to human CD11b (Dominguez et al. 2001)Mouse anti pig CD11R1, clone MIL4 is cross reactive with the guinea pig and is useful for the identification of a population of guinea pig natural killer cells, Kurloff cells (Takizawa et al. 2004) (Eremin et al. 1980).