Mouse monoclonal [WM15] antibody to CD13 (PE-Cyanine 7).
Specificity
This antibody recognises an extracellular epitope of human CD13 cell surface glycoprotein, a 150 kDa molecule expressed on granulocytes, endothelial cells, epithelial cells, and myeloid progenitors.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human AML cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
WM15
Isotype
IgG1
Conjugate
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
150 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M, AP-N, APN, gp150, hAPN, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN
Separation of human CD13 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD13 Antibody [WM15] (PE-Cyanine 7) (4 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD13
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.