Anti-CD130 / gp130 Antibody [B-T2] (A279869)

Anti-CD130 / gp130 Antibody [B-T2]

Mouse monoclonal [B-T2] antibody to CD130 / gp130.

This antibody recognises soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type and multiple fibronectin type-III domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain. Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4+ T cells(de Groot et al. 2012, Pflanz et al. 2004).

Flow Cytometry, ELISA

Flow Cytometry: 0.2ug/10⁶cells, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Human

Natural soluble gp130 receptor.

Mouse

Monoclonal

B-T2

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD130 antibody, clone B-T2 recognizes soluble and membrane bound gp130 receptor, also known as CD130, Interleukin-6 receptor subunit beta, Oncostatin-M receptor subunit alpha or Interleukin-6 signal transducer. CD130 is a 918 amino acid, ~130 kDa single pass type-1 transmembrane glycoprotein containing a single Ig-like C2 type and multiple fibronectin type-III domains. CD130 can be cleaved to form a monomeric soluble ~100 kDa form of the receptor detectable in plasma and other biologic fluids where it acts as an IL-6 antagonist (Müller-Newen et al. 1998). Mouse anti Human CD130 antibody, clone B-T2 binds to an epitope dependent at least partially on the presence of the Ig-like domain (Hammacher et al. 1998).Mouse anti Human CD130 antibody, clone B-T2 has been reported to block receptor activity and inhibit IL-6 induced proliferation of XG1 cells and IL-27 mediated proliferation of naive CD4+ T cells(de Groot et al. 2012, Pflanz et al. 2004).