Anti-CD14 Antibody (A84169)

CD14 expression in Human Lymph node (A) + peptide (B) and Tonsil (C) + peptide (D) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CD14 Antibody (A84169) at 2µg/ml and detected by chemiluminescence.

CD14 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-CD14 Antibody (A84169) at 8µg/ml and revealed with horseradish peroxidase (HRP).

Negative control for CD14 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

CD14 expression in A549 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-CD14 Antibody (A84169) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.