Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (A121741)

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This product is discontinued

Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (A121741) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488)

Description

Mouse monoclonal [55-7H1] antibody to CD144 (Alexa Fluor® 488).

Specificity

This antibody recognizes a calcium-independent extracellular epitope on CD144, an adhesion molecule expressed on endothelial cells.

Applications

Flow Cytometry, WB, ICC

Dilutions

Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human

Immunogen

Human endothelial cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

55-7H1

Isotype

IgG1

Conjugate

Alexa Fluor® 488

Excitation: 490nm, Emission: 525nm

Purification

This antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

7B4 antigen, Cadherin-5, CDH5, Vascular endothelial cadherin

Isotype Controls

Mouse IgG1 [MOPC-21] (Alexa Fluor® 488) (A18720)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (A121741)

Flow cytometric analysis of HUVEC (human umbilical vein endothelial cells) stained with Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (4µl reagent per milion cells in 100µl of cell suspension).

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Flow Cytometry - Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (A121741)

Separation of HUVEC cells (red-filled) from HL-60 cells (black-dashed) in flow cytometry analysis using Anti-CD144 Antibody [55-7H1] (Alexa Fluor® 488) (4µl reagent per milion cells in 100µl of cell suspension).