Mouse monoclonal [3G8] antibody to CD16 (Alexa Fluor® 700).
Specificity
This antibody recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes, and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 106 cells in a suspension.
Reactivity
Human, Non-Human Primates
Immunogen
Human neutrophils.
Host
Mouse
Clonality
Monoclonal
Clone ID
3G8
Isotype
IgG1
Conjugate
Alexa Fluor® 700
Excitation: 702nm, Emission: 723nm
Purification
This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.
Storage
Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD16-II, CD16a, CD16a antigen, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG3, FcgammaRIIIA, FCGR3, FCGR3A, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A