Anti-CD16 Antibody [KD1] (PE) (A280810)

Anti-CD16 Antibody [KD1] (PE)

Mouse monoclonal [KD1] antibody to CD16 (PE).

This antibody recognises human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages , whereas CD16B is primarily expressed by granulocytes. Clone KD1 recognises both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine and ovine.

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human, Bovine, Sheep, Dolphin

This antibody does not cross react with Rat or Dog

A polyclonal population of NK cells.

Mouse

Monoclonal

KD1

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD16 antibody, clone KD1 recognizes human CD16, a 50-65 kDa cell surface molecule, which is the low affinity receptor for IgG (FcR III). CD16 exists as a transmembranous form (Fc gammaRIIIA, or CD16A) and a glycosyl phosphatidylinositol (GPI) anchored form (Fc gammaRIIIB, or CD16B). CD16A is expressed by NK cells, some T cells, and macrophages (Moretta et al. 1990), whereas CD16B is primarily expressed by granulocytes (Bonecchi et al. 1999). Clone KD1 recognizes both forms of CD16 and will therefore recognize all cell types expressing CD16. Mouse anti Human CD16, clone KD1 can be used to identify CD16 in a range of species including bovine (Boysen et al. 2010) and ovine (Elhmouzi-Younes et al. 2010).

CD16-II, CD16a, CD16a antigen, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, FCG3, FcgammaRIIIA, FCGR3, FCGR3A, FcR-10, FcRIII, FcRIIIa, IGFR3, IgG Fc receptor III-2, IgG Fc receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-A

This product is for research use only. It is not intended for diagnostic or therapeutic use.