Anti-CD16 + CD32 Antibody [FCR4G8] (A280460)

Anti-CD16 + CD32 Antibody [FCR4G8]

Rat monoclonal [FCR4G8] antibody to CD16 + CD32.

This antibody recognises an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.

Flow Cytometry: 1:25 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

PU5 1.8 IOE7 Balb/c mouse cell line.

Rat

Monoclonal

FCR4G8

IgG2b

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.