Mouse monoclonal [HP-3G10] antibody to CD161 (APC-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD161, a type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes, and a subset of T cells as a disulphide-linked homodimer.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human NK cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
HP-3G10
Isotype
IgG1
Light Chains
kappa
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
C-type lectin domain family 5 member B, CLEC5B, HNKR-P1a, Killer cell lectin-like receptor subfamily B member 1, KLRB1, Natural killer cell surface protein P1A, NKR-P1A, NKRP1A
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD161 Antibody [HP-3G10] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Flow cytometric analysis of human lymphocytes stained using Anti-CD161 Antibody [HP-3G10] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood) and Anti-CD4 Antibody [MEM-241] (FITC) (20µl reagent per 100µl of peripheral whole blood).
Separation of human CD161 positive CD4 negative lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD161 Antibody [HP-3G10] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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Proteins predicted to interact with CD161
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
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