Mouse monoclonal [GHI/61] antibody to CD163 (PerCP-Cyanine 5.5).
Specificity
This antibody recognizes an extracellular epitope of CD163, an approximately 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Hairy cell leukemia cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
GHI/61
Isotype
IgG1
Light Chains
kappa
Conjugate
PerCP-Cyanine 5.5
Excitation: 482nm, Emission: 695nm
Purification
This antibody is conjugated with tandem dye PerCP-Cyanine 5.5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
130 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Hemoglobin scavenger receptor, M130, Scavenger receptor cysteine-rich type 1 protein M130
Flow cytometric analysis of human peripheral blood with Anti-CD163 Antibody [GHI/61] (PerCP-Cyanine 5.5).
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Proteins predicted to interact with CD163
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.