Mouse monoclonal [MEM-166] antibody to CD177 (PE).
Specificity
This antibody reacts with CD177, a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-166
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD177 antigen, HNA-2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, PRV1
Separation of human CD177 positive neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD177 with Anti-CD177 Antibody [MEM-166] (PE) (A85950), (20 µl reagent / 100 µl of peripheral whole blood).
Publishing research using Anti-CD177 Antibody [MEM-166] (PE) (A85950)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with CD177
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_1.jpg?profile=product_top)
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_2.jpg?profile=product_top)
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_1.jpg?profile=product_image)
![Flow Cytometry - Anti-CD177 Antibody [MEM-166] (PE) (A85950)](https://cdn.antibodies.com/image/catalog/85/A85950_2.jpg?profile=product_image)