Mouse monoclonal [IVA35] antibody to CD18 (Alexa Fluor® 488).
Specificity
This antibody reacts with an extracellular epitope of CD18 antigen (bovine), a 90-95 kDa type I transmembrane protein (integrin) expressed on all leukocytes.
Applications
ICC, Flow Cytometry
Dilutions
Flow Cytometry: 2-5 µg/ml
Reactivity
Bovine, Human
Immunogen
Bovine leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
IVA35
Isotype
IgG1
Conjugate
Alexa Fluor® 488
Excitation: 490nm, Emission: 525nm
Concentration
500 µg/ml
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Separation of human neutrophil granulocytes (red-filled) from human CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD18 Antibody [IVA35] (Alexa Fluor® 488) (concentration in sample is 5 µg/ml).
Separation of bovine leukocytes stained with Anti-CD18 Antibody [IVA35] (Alexa Fluor® 488) (concentration in sample is 3 µg/ml, red-filled) from bovine leukocytes stained using Mouse IgG1 Isotype Control [MOPC-21] (Alexa Fluor® 488) (concentration in sample is 3 µg/ml, black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood.
Flow cytometry surface staining pattern of human peripheral whole blood stained with Anti-CD18 Antibody [IVA35] (Alexa Fluor® 488) (concentration in sample is 5 µg/ml).
Proteins predicted to interact with CD18
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.