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Anti-CD1w2 Antibody [CC20] (PE) (A280013)

Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013) - Antibodies.com Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013) - Antibodies.com
$730
Product Datasheet

Mouse monoclonal [CC20] antibody to CD1w2 (PE) for Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.

  • Specifications

Name
Anti-CD1w2 Antibody [CC20] (PE)
Description
Mouse monoclonal [CC20] antibody to CD1w2 (PE).
Specificity
This antibody recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Bovine, Sheep, Goat, Dog, Horse, Cat
Host
Mouse
Clonality
Monoclonal
Clone ID
CC20
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD1w2 antibody, clone CC20 recognises the bovine CD1w2 cell surface antigen, a glycoprotein heterodimer of ~12 kDa and ~46 kDa. CD1w2 is expressed by dendritic cells, cortical thymocytes and a minority of medullary thymocytes, with a pattern similar to antibodies of the CD1b cluster in humans.
Isotype Controls
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013)

Figure A. Anti-MHC Class II Monomorphic Antibody [IL-A21] (FITC) (A280726) and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. Anti-MHC Class II Monomorphic Antibody [IL-A21] (FITC) (A280726) and Anti-CD1w2 Antibody [CC20] (PE) (A280013). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-CD1w2 Antibody [CC20] (PE) (A280013)

Figure A. Anti-CD4 Antibody [CC8] (FITC) (A280063) and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. Anti-CD4 Antibody [CC8] (FITC) (A280063) and Anti-CD1w2 Antibody [CC20] (PE) (A280013). All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes. Data acquired on the ZE5 Cell Analyzer.
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