This antibody recognises the bovine homologue of human CD2.Clone CC42 inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Bovine, Goat
Host
Mouse
Clonality
Monoclonal
Clone ID
CC42
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Bovine CD2, clone CC42, recognizes the bovine homologue of human CD2.Clone CC42 inhibits rosetting with SRBC and stains cells in primary and secondary lymphoid organs in patterns consistent with those seen by human CD2 monoclonal antibodies.
Figure A. Alexa Fluor 647 conj+I122ugated mouse anti bovine CD4 and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Alexa Fluor 647 conjugated mouse anti bovine CD4 and Anti-CD2 Antibody [CC42] (FITC) (A280048). All experiments performed on red cell lysed bovine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Figure A. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Anti-CD4 Antibody [CC8] (PE) (A280064). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-CD4 Antibody [CC8] (PE) (A280064) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD4 Antibody [CC8] (PE) (A280064) and Anti-CD2 Antibody [CC42] (FITC) (A280048). All experiments performed on red cell lysed bovine blood gated on mononuclear cells
Figure A. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Mouse IgG2a (PE) - Isotype Control (A282644). Figure B. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Anti-CD8 Antibody [CC63] (PE) (A280058). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-CD8 Antibody [CC63] (PE) (A280058) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-CD8 Antibody [CC63] (PE) (A280058) and Anti-CD2 Antibody [CC42] (FITC) (A280048). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-NCR1 Antibody [AKS1] (PE) (A279671) and Mouse IgG1 (FITC) - Isotype Control (A282453). Figure B. Anti-NCR1 Antibody [AKS1] (PE) (A279671) and Anti-CD2 Antibody [CC42] (FITC) (A280048). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.
Figure A. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Mouse IgG1 (PE) - Isotype Control (A282643). Figure B. Anti-CD2 Antibody [CC42] (FITC) (A280048) and Anti-NCR1 Antibody [AKS1] (PE) (A279671). All experiments performed on red cell lysed bovine blood gated on mononuclear cells.