Anti-CD2 Antibody [Mac288] (A281018)

Anti-CD2 Antibody [Mac288]

Rat monoclonal [Mac288] antibody to CD2.

This antibody recognises equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type and Ig-like V-type domains.Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex. recognises CD2 in Haflinger and Lusitano horse breeds. Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry.

IHC-Fr, Flow Cytometry

Flow Cytometry: 1:200, Use 50µl of the suggested working dilution to label 7.5 x 105 cells in 100µl

Horse

This antibody does not cross react with Pig

Equine peripheral blood lymphocytes.

Rat

Monoclonal

Mac288

IgG2a

Lot Specific

Tissue Culture Supernatant

Liquid

Supplied in 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Horse CD2 antibody, clone Mac288 recognizes equine CD2, a 347 amino acid, single pass type-1 transmembrane glycoprotein containing single Ig-like C2-type and Ig-like V-type domains.Equine CD2 is expressed by a large proportion of peripheral blood lymphocytes, most T-cells and few B-cells, it is not present on granulocytes. Immunohistochemical staining shows expression in most T cells of lymph node paracortex and occasional staining of lymphocytes in the follicular areas. In the thymus, rat anti horse CD2 antibody, clone Mac288 stains the majority of mature thymocytes in the medulla and at least half of the immature thymocytes in the cortex (Kydd et al. 1994). Rat anti Horse CD2 antibody, clone Mac288 recognizes CD2 in Haflinger and Lusitano horse breeds (Ferreira-Dias et al. 2005). Rat anti Horse CD2 antibody, clone Mac288 is a reliable pan T-cell marker for analysis of cell mediated immunity in horses infected with equine herpes virus by multi-parameter flow cytometry (Platt et al. 2010).

Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5