Anti-CD20 Antibody [FMC7] (FITC) (A280482)

Anti-CD20 Antibody [FMC7] (FITC)

Mouse monoclonal [FMC7] antibody to CD20 (FITC).

This antibody recognises a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20. Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Human

HRIK cells - human B-Lymphoblastoid line.

Mouse

Monoclonal

FMC7

IgM

Gel filtration of ascites.

Lot Specific

Liquid

Supplied in TRIS buffered glycine with 0.1% Sodium Azide and 0.2% BSA.

Shipped at ambient temperature. Store at 4°C. This product is photosensitive and should be protected from light.

Mouse anti Human B cells antibody, clone FMC7 recognizes a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. Mouse anti Human B cells antibody, clone FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukemia and hairy cell leukemia.The expression pattern of the FMC7 antigen closely corresponds to that seen with CD22. Mouse anti Human B cells antibody, clone FMC7 recognizes a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20 (Serke et al. 2001). Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent (Polyak et al. 2003).

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1

This product is for research use only. It is not intended for diagnostic or therapeutic use.