Mouse oligoclonal [L26 + IGEL/773] antibody to CD20 (CF®740).
Applications
Flow Cytometry (Cell Surface), IF, IHC, IHC-P
Positive Control
Daudi, Raji, U266, and human lymphocytes or lymph node and tonsil tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human, Monkey, Baboon
Cross Reactivity
No cross-reactivity with Rat, Bovine, Canine or Porcine. Cross-reactivity with other species has not been determined.
Immunogen
Clone L26: Human tonsil B cells. Clone IGEL/773: Recombinant human CD20 protein.
Host
Mouse
Clonality
Oligoclonal
Clone ID
L26 + IGEL/773
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®740
Concentration
0.1 mg/mL
Predicted MW
33-37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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