This antibody recognises the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation. Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.
Applications
IP, Flow Cytometry, IHC-Fr, IF, Electron Microscopy
Dilutions
Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
Rat Thymocyte Membrane Glycoproteins.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-2
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.The CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation. Mouse anti Rat CD200 antibody, clone OX-2 blocks OX-2 mediated T cell co-stimulation (Ko et al. 2009).Mouse anti Rat CD200 antibody, clone OX-2 is routinely tested by flow cytometry on rat thymocytes.
