Rat monoclonal [OX-90] antibody to CD200 / OX2 (Biotin).
Specificity
This antibody recognises the mouse CD200 cell surface antigen, also known as OX2.CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
Applications
Flow Cytometry
Dilutions
Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse CD200-Rat CD4 fusion protein.
Host
Rat
Clonality
Monoclonal
Clone ID
OX-90
Isotype
IgG2a
Conjugate
Biotin
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2.CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
