Rat monoclonal [OX-90] antibody to CD200 / OX2 (PE).
Specificity
This antibody recognises the mouse CD200 cell surface antigen, also known as OX2.CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
Applications
Flow Cytometry
Dilutions
Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse CD200-Rat CD4 fusion protein.
Host
Rat
Clonality
Monoclonal
Clone ID
OX-90
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD200 antibody, clone OX-90 recognizes the mouse CD200 cell surface antigen, also known as OX2.CD200 is expressed by splenic B lymphocytes, follicular dendritic cells, splenic endothelium and by neurons.The CD200 - CD200 ligand system is of importance in the control of macrophage and granulocyte activation.
