Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (A121753)

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This product is discontinued

Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (A121753) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD206 Antibody [15-2] (Alexa Fluor® 647)

Description

Mouse monoclonal [15-2] antibody to CD206 (Alexa Fluor® 647).

Specificity

This antibody (also known as MR15-2) recognizes an extracellular epitope of CD206, a 162-175 kDa type I transmembrane protein expressed mainly on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes.

Applications

Flow Cytometry, WB, ICC

Dilutions

Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human

Immunogen

Purified human mannose receptor.

Host

Mouse

Clonality

Monoclonal

Clone ID

15-2

Isotype

IgG1

Conjugate

Alexa Fluor® 647

Excitation: 650nm, Emission: 665nm

Purification

This antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, CLEC13D, CLEC13DL, hMR, Human mannose receptor, Macrophage mannose receptor 1, Macrophage mannose receptor 1-like protein 1, MMR, MRC1, MRC1L1

Isotype Controls

Mouse IgG1 [MOPC-21] (Alexa Fluor® 647) (A18746)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Flow Cytometry - Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (A121753)

Flow cytometric analysis of human stimulated monocytes (GM-CSF + IL-4) stained with Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (4µl reagent per milion cells in 100µl of cell suspension).

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Flow Cytometry - Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (A121753)

Flow cytometric analysis of human stimulated monocytes (GM-CSF + IL-4) using Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (4µl reagent per milion cells in 100µl of cell suspension) and Anti-CD11c Antibody [BU15] (PE) (20µl reagent per milion cells in 100µl of cell suspension).

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Flow Cytometry - Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (A121753)

Separation of human CD206 and CD11c positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis of human stimulated monocytes (GM-CSF + IL-4) stained with Anti-CD206 Antibody [15-2] (Alexa Fluor® 647) (4µl reagent / 100µl of peripheral whole blood).