Anti-CD25 Antibody [IL2RA/2395] (CF®488A) (A353938)

Name: Anti-CD25 Antibody [IL2RA/2395] (CF®488A)
Brand: Antibodies.com
SKU: A353938
Price: 275.0 GBP
Availability: InStock

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$370

Mouse monoclonal [IL2RA/2395] antibody to CD25 (CF®488A) for IHC and IHC-P .

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Tags:

B Cell Marker Basophil Marker Cytotoxic T cell Marker Mast Cell Marker

Specifications

Name

Anti-CD25 Antibody [IL2RA/2395] (CF®488A)

Description

Mouse monoclonal [IL2RA/2395] antibody to CD25 (CF®488A).

Applications

IHC, IHC-P

Positive Control

PHA-stimulated human lymphocytes and human lymph node or tonsil tissue.

Dilutions

IHC-P: 2-4 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.

Reactivity

Human

Immunogen

Recombinant fragment of human CD25 (IL2RA) protein corresponding to approximately amino acids 42-183 (exact sequence is proprietary).

Host

Mouse

Clonality

Monoclonal

Clone ID

IL2RA/2395

Isotype

IgG2b

Conjugate

CF®488A

Concentration

0.1 mg/mL

Predicted MW

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Storage

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Synonyms

IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, IL2RA, Interleukin-2 receptor subunit alpha, p55, TAC antigen

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Immunohistochemistry - Anti-CD25 Antibody [IL2RA/2395] (CF®488A) (A353938)

Representative staining of formalin-fixed, paraffin-embedded human tonsil using the unconjugated form of Anti-CD25 Antibody [IL2RA/2395].

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SDS-PAGE - Anti-CD25 Antibody [IL2RA/2395] (CF®488A) (A353938)

Analysis of the unconjugated form of Anti-CD25 Antibody [IL2RA/2395]. Protein integrity and purity were assessed by SDS–PAGE under reducing conditions.

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Anti-CD25 Antibody [IL2RA/2395] (CF®488A) (A353938)

The protein microarray results shown are representative of the unconjugated form of the Anti-CD25 Antibody [IL2RA/2395] (CF®488A). Protein microarray analysis of more than 19,000 full-length human proteins was performed using the Anti-CD25 Antibody [IL2RA/2395] (CF®488A). The Z-score reflects antibody binding intensity to each protein on the HuProt™ array and is expressed as the number of standard deviations above the mean array signal. The S-score represents the difference in Z-score between adjacent ranked proteins and provides a measure of target specificity. Antibodies with an S-score of at least 2.5 are considered highly specific for their intended target.

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