Mouse monoclonal [IL2RA/2395] antibody to CD25 (CF®647).
Applications
IHC, IHC-P
Positive Control
PHA-stimulated human lymphocytes and human lymph node or tonsil tissue.
Dilutions
IHC-P: 2-4 µg/mL for 30 minutes at room temperature. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Recombinant fragment of human CD25 (IL2RA) protein corresponding to approximately amino acids 42-183 (exact sequence is proprietary).
Host
Mouse
Clonality
Monoclonal
Clone ID
IL2RA/2395
Isotype
IgG2b
Conjugate
CF®647
Excitation: 650nm, Emission: 665nm
Concentration
0.1 mg/mL
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
