Mouse monoclonal [MIH26] antibody to CD272/BTLA (PE).
Specificity
This antibody recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Cells transfected with human CD272.
Host
Mouse
Clonality
Monoclonal
Clone ID
MIH26
Isotype
IgG2a
Light Chains
kappa
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of human CD272 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD272/BTLA with Anti-CD272/BTLA Antibody [MIH26] (PE) (A320641), (10 µl reagent / 100 µl of peripheral whole blood).
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