Mouse monoclonal [C28/74] antibody to CD28 (CF®647).
Applications
Flow Cytometry, IHC
Positive Control
Jurkat cells; human lymph node and tonsil tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human
Immunogen
Recombinant human CD28 protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
C28/74
Isotype
IgG1
Light Chains
kappa
Conjugate
CF®647
Excitation: 650nm, Emission: 665nm
Concentration
0.1 mg/mL
Predicted MW
44 kDa (monomer); 88 kDa (dimer)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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