Anti-CD3 epsilon Antibody [SK7] (FITC) (A320261)

Anti-CD3 epsilon Antibody [SK7] (FITC)

Mouse monoclonal [SK7] antibody to CD3 epsilon (FITC).

This antibody recognises the T-cell surface glycoprotein CD3 epsilon chain (CD3?), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3? is a subunit of the TCR-CD3 cell surface receptor complex, which is expressed at the surface of mature T cells, NKT cells and thymocytes. The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell. Deficiency of the CD3e chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency (SCID) phenotype. Mouse anti-human CD3, clone SK7 binding to CD3 has been shown to be blocked by clone OKT3 and 12F6.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Human

Human CD3 epsilon.

Mouse

Monoclonal

SK7

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

>90% (by SDS-PAGE).

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human CD3 antibody, clone SK7 recognizes the T-cell surface glycoprotein CD3 epsilon chain (CD3?), also known as T-cell surface antigen T3/Leu-4 epsilon. CD3? is a subunit of the TCR-CD3 cell surface receptor complex, which is expressed at the surface of mature T cells, NKT cells and thymocytes (Mariuzza et al. 2020). The CD3 complex is required for intracellular signaling and for TCR surface expression. Recruitment of the TCR/CD3 complex initiates early events leading to proliferation and differentiation of mature T cells into an effector cell (Guy & Vignali 2009).Deficiency of the CD3e chain contributes to blocking T-cell development and presentation of a severe combined immunodeficiency (SCID) phenotype (Fischer et al. 2005).Mouse anti-human CD3, clone SK7 binding to CD3 has been shown to be blocked by clone OKT3 and 12F6 (Li et al. 2005).

CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

This product is for research use only. It is not intended for diagnostic or therapeutic use.