Rabbit polyclonal antibody to CD3 ζ (Phospho-Tyr142)
Specificity
The antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142.
Applications
WB
Dilutions
Western blotting: 1:500~1:1000
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y(p)-Q-G) derived from Human CD3 ζ.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody #11655. The lane on the left is treated with antigen-specific peptide.
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