Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647) (A121725)

This product is discontinued

Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647) (A121725) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647)

Description

Mouse monoclonal [EM-17] antibody to CD3 zeta (phospho Tyr153) (Alexa Fluor® 647).

Specificity

This antibody recognizes phosphorylated intracellular tyrosine 153 of CD3 zeta chain, which is a component of TCR/CD3 complex expressed on T cells.

Applications

Flow Cytometry

Dilutions

Flow Cytometry: 2-6 µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to mouse CD3 zeta (phospho Tyr153), conjugated to KLH.

Host

Mouse

Clonality

Monoclonal

Clone ID

EM-17

Isotype

IgG1

Conjugate

Alexa Fluor® 647

Excitation: 650nm, Emission: 665nm

Purification

This antibody is conjugated with Alexa Fluor® 647 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Concentration

0.1 mg/ml

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

CD247, CD3Z, T-cell receptor T3 zeta chain, T-cell surface glycoprotein CD3 zeta chain, T3Z, TCRZ

Isotype Controls

Mouse IgG1 [MOPC-21] (Alexa Fluor® 647) (A18746)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Enlarge Image

Flow Cytometry - Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647) (A121725)

Flow cytometric analysis of Jurkat cell suspension labeling CD3 zeta (phospho Tyr153) with Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647). Concentration in sample 3µg/ml.

Enlarge Image

Flow Cytometry - Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647) (A121725)

Separation of JURKAT cells stained with Anti-CD3 zeta (phospho Tyr153) Antibody [EM-17] (Alexa Fluor® 647) (concentration in sample 3µg/ml, red-filled) from Jurkat cells stained with mouse IgG1 isotype control [MOPC-21] (Alexa Fluor® 647) (concentration in sample 3 µg/ml, black-dashed) in flow cytometry analysis of Jurkat cell suspension.