Dilutions
WB: 2 - 5 µg/ml; Positive Control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, Non-reducing conditions recommended, Flow Cytometry: Intracellular staining: 1-9 µg/ml; Positive Control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.