Mouse monoclonal [UP-H2] antibody to CD300e (FITC).
Specificity
This antibody recognizes an extracellular epitope on CD300e / IREM-2, a 32 kDa glycoprotein expressed by mature monocytes and peripheral blood myeloid dendritic cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
CD300e-HA-transfected cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
UP-H2
Isotype
IgG1
Light Chains
kappa
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
32 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD300 antigen-like family member E, CD300LE, CLM-2, CLM2, CMRF35-A5, CMRF35-like molecule 2, CMRF35A5, Immune receptor expressed on myeloid cells 2, IREM-2, IREM2, PIgR-2, PIgR2, Poly-Ig receptor 2, Polymeric immunoglobulin receptor 2
Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD300e with Anti-CD300e Antibody [UP-H2] (FITC) (A320708), (4 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with CD300e
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.