Mouse monoclonal [WM53] antibody to CD33 (APC-Cyanine 7).
Specificity
This antibody reacts with an extracellular epitope of CD33, a 67 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily, expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells, and mast cells. It is absent on platelets, lymphocytes, erythrocytes, and hematopoietic stem cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human AML cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
WM53
Isotype
IgG1
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
67 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!