This antibody recognises the murine CD38 cell surface antigen, a ~42-46kDa glycoprotein expressed by B lymphocytes, a proportion of peripheral T lymphocytes and by peritoneal macrophages.In the mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse pre-B cells derived from IL-7 dependent bone marrow cultures.
Host
Rat
Clonality
Monoclonal
Clone ID
90
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Mouse CD38 antibody, clone 90 recognizes the murine CD38 cell surface antigen, a ~42-46kDa glycoprotein expressed by B lymphocytes, a proportion of peripheral T lymphocytes and by peritoneal macrophages.In the mouse CD38 is expressed by follicular B cells, is down regulated on germinal centre B cells, and not expressed by mature plasma cells. This pattern of expression contrasts strikingly with that seen in the human.