Anti-CD4 Antibody (A91607)

Western blot analysis of extracts of Rat lung, using Anti-CD4 Antibody (A91607) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Western blot analysis of extracts of Mouse spleen, using Anti-CD4 Antibody (A91607) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Western blot analysis of extracts of THP-1 cells, using Anti-CD4 Antibody (A91607) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of rat spleen using Anti-CD4 Antibody (A91607) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).