Mouse monoclonal [CD4/3027] antibody to CD4 (Biotin).
Applications
Functional Studies
Positive Control
Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60 cells. Lymph node or tonsil tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/mL, WB: 1-2 µg/mL, IHC-P: 1-2 µg/mL. Directly conjugated primary antibodies may require increased working concentrations compared with indirect detection systems. Antibody concentration and assay conditions should be optimized for each application. A suggested starting concentration is 1-2 µg/mL for most assays.
Reactivity
Human
Immunogen
Recombinant extracellular domain fragment of human CD4 corresponding to approximately amino acids 245-392 (exact sequence is proprietary).
Host
Mouse
Clonality
Monoclonal
Clone ID
CD4/3027
Isotype
IgG2b
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
The protein microarray results shown are representative of the unconjugated form of the Anti-CD4 Antibody [CD4/3027] (Biotin). Protein microarray analysis of more than 19,000 full-length human proteins was performed using the Anti-CD4 Antibody [CD4/3027] (Biotin). The Z-score reflects antibody binding intensity to each protein on the HuProt™ array and is expressed as the number of standard deviations above the mean array signal. The S-score represents the difference in Z-score between adjacent ranked proteins and provides a measure of target specificity. Antibodies with an S-score of at least 2.5 are considered highly specific for their intended target.
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