Anti-CD4 Antibody [SK3] (APC) (A352001)

Name: Anti-CD4 Antibody [SK3] (APC)
Brand: Antibodies.com
SKU: A352001
Price: 230.0 GBP
Availability: InStock

$310

Mouse monoclonal [SK3] antibody to CD4 (APC) for Flow Cytometry (Cell Surface).

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-9 business days.

Tags:

Endosomal Marker Regulatory T Cell Marker T Follicular Helper Cell Marker T Helper Cell Marker

Specifications

Name

Anti-CD4 Antibody [SK3] (APC)

Description

Mouse monoclonal [SK3] antibody to CD4 (APC).

Applications

Flow Cytometry (Cell Surface)

Positive Control

Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM, and HL-60 cells.

Dilutions

Flow Cytometry: 5 µL/million cells in 100 µL.

Reactivity

Human

Immunogen

Human peripheral blood T cells.

Host

Mouse

Clonality

Monoclonal

Clone ID

SK3

Isotype

IgG1

Light Chains

kappa

Conjugate

APC

Excitation: 645nm, Emission: 660nm

Concentration

50 µg/mL

Predicted MW

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.

Storage

Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.

Synonyms

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Isotype Controls

Mouse IgG1 [MOPC-21] (APC) (A86198)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (21)

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®700) (teal). Fluorescence was detected in the R712 (APC-700) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®405M) (blue). Fluorescence was detected in the V450 (Pacific Blue) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®405S) (blue). Fluorescence was detected in the V450 (Pacific Blue) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®405L) (green). Fluorescence was detected in the V525 (FITC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®488A) (green). Fluorescence was detected in the B525 (FITC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®503R) (green). Fluorescence was detected in the B525 (FITC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®514) (green). Fluorescence was detected in the B525 (FITC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Representative flow cytometry analysis of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (R-PE) ( Orange). Unstained lymphocyte-gated PBMCs (gray) served as the negative control. Fluorescence was detected in the Y585 (PE) channel.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®568) (orange). Fluorescence was detected in the Y585 (PE) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®575) (orange). Fluorescence was detected in the Y585 (PE) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (RPE-Astral™616) (red). Fluorescence was detected in the B610 (PI) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®647Plus) (pink). Fluorescence was detected in the R660 (APC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®660C) (pink). Fluorescence was detected in the R712 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (APC) (pink). Fluorescence was detected in the R660 (APC) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (APC-Astral™813) (teal). Fluorescence was detected in the R840 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®820) (teal). Fluorescence was detected in the IR840 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (PerCP) (magenta). Fluorescence was detected in the B690 (PerCP) channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (RPE-Astral™775) (teal). Fluorescence was detected in the Y763 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Anti-CD4 Antibody [SK3] (APC) (A352001)

Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD4 Antibody [SK3] (CF®870) (teal). Fluorescence was detected in the IR885 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.

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Immunofluorescence - Anti-CD4 Antibody [SK3] (APC) (A352001)

Immunofluorescence staining of 6×His-tagged CD4 in HeLa cells transiently transfected with a CD4-6×His expression vector. Following fixation with 4% paraformaldehyde (PFA), cells were stained with Anti-CD4 Antibody [SK3] (CF®488A; green) and Anti-His Tag Antibody [2405.H6] (CF®680; magenta). Nuclei were counterstained with Hoechst 33342 (blue). Images were acquired by confocal microscopy.

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