Anti-CD4 Antibody [YKIX302.9] (PE-Cyanine 7) (A282043)

Anti-CD4 Antibody [YKIX302.9] (PE-Cyanine 7)

Rat monoclonal [YKIX302.9] antibody to CD4 (PE-Cyanine 7).

This antibody recognises the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model. Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Canine

Canine concanavilin A activated T cell blasts.

Rat

Monoclonal

YKIX302.9

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.

Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Moore et al. 1992).

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

This product is for research use only. It is not intended for diagnostic or therapeutic use.