This antibody reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d.
Applications
Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1-4 µg/ml
Reactivity
Human, Non-Human Primates
Immunogen
Peripheral blood mononuclear cells of a patient suffering with CLL.
Host
Mouse
Clonality
Monoclonal
Clone ID
HIP1
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
135-145 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Separation of human thrombocytes (red-filled) from CD42b negative lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling CD42b with Anti-CD42b Antibody [HIP1] (A85789), (concentration in sample 4 µg/ml) GAM APC.
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