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Anti-CD45 Antibody [136-4B5] (CF®740) (A352259)

Mouse monoclonal [136-4B5] antibody to CD45 (CF®740) for Flow Cytometry (Cell Surface), IF, IHC and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-9 business days.
Name
Anti-CD45 Antibody [136-4B5] (CF®740)
Description
Mouse monoclonal [136-4B5] antibody to CD45 (CF®740).
Applications
Flow Cytometry (Cell Surface), IF, IHC, IHC-P
Positive Control
Ramos, U-698 and GA-10 cells or tonsil tissue.
Dilutions
Flow Cytometry: 1-2 µg/million cells, IF: 0.5-1 µg/mL, IHC-P. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Immunogen
Stimulated human leukocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
136-4B5
Isotype
IgG1
Light Chains
kappa
Conjugate

CF®740

Concentration
0.1 mg/mL
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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