Mouse monoclonal [2D1] antibody to CD45 (RPE-Astral™775).
Applications
Flow Cytometry (Cell Surface)
Positive Control
Human peripheral blood mononuclear cells (PBMCs).
Dilutions
Flow Cytometry: 5 µL/million cells in 100 µL.
Reactivity
Human
Immunogen
Human peripheral blood mononuclear cells (PBMCs).
Host
Mouse
Clonality
Monoclonal
Clone ID
2D1
Isotype
IgG1
Light Chains
kappa
Conjugate
RPE-Astral™775
Concentration
100 µg/mL
Predicted MW
180 - 240 kDa (multiple isoforms)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% recombinant BSA (produced in CHO cells) and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (CF®405M) (). Fluorescence was detected in the V450 (Pacific Blue) channel. Isotype control (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (CF®488A) (). Fluorescence was detected in the B525 (FITC) channel. Isotype control (gray) is shown for comparison.
Representative flow cytometry analysis of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (R-PE) ( Orange). Unstained lymphocyte-gated PBMCs (gray) served as the negative control. Fluorescence was detected in the Y585 (PE) channel.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (RPE-Astral™616) (Red). Fluorescence was detected in the B610 (PI) channel. Isotype control antibody conjugated to RPE-Astral™616 (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (CF®647Plus) (). Fluorescence was detected in the R660 (APC) channel. Isotype control (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (APC) (Pink). Fluorescence was detected in the R660 (APC) channel. Isotype control antibody conjugated to APC (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (APC-Astral™813) (Teal). Fluorescence was detected in the R840 channel. Isotype control antibody conjugated to APC-Astral™813 (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (CF®820) (teal). Fluorescence was detected in the R840 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (PerCP) (Magenta). Fluorescence was detected in the B690 (PerCP) channel. Isotype control antibody conjugated to PerCP (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (RPE-Astral™775) (Teal). Fluorescence was detected in the Y763 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
Flow cytometry of lymphocyte-gated PBMCs stained with Anti-CD45 Antibody [2D1] (CF®870) (teal). Fluorescence was detected in the IR885 channel. Unstained lymphocyte-gated PBMCs (gray) is shown for comparison.
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_1.jpg?profile=product_top)
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![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_3.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_4.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_5.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_6.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_7.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_8.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_9.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_10.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_11.jpg?profile=product_top)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_1.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_2.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_3.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_4.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_5.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_6.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_7.jpg?profile=product_top_thumb)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_1.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_2.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_3.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_4.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_5.jpg?profile=product_image)
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![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_7.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_8.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_9.jpg?profile=product_image)
![Flow cytometry - Anti-CD45 Antibody [2D1] (RPE-Astral™775) (A352043) - Antibodies.com](https://cdn.antibodies.com/image/catalog/352/A352043_10.jpg?profile=product_image)
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