Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (A18760)

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This product is discontinued

Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (A18760) has been discontinued and is no longer available.

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Specifications

Name

Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700)

Description

Mouse monoclonal [MEM-28] antibody to CD45 (Alexa Fluor® 700).

Specificity

This antibody reacts with an extracellular epitope on all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.

Applications

Flow Cytometry, WB, ICC

Dilutions

Flow Cytometry: This reagent is designed for analysis of human blood cells using 4µl reagent / 100µl of whole blood or 10⁶ cells in a suspension.

Reactivity

Human

Cross Reactivity

This antibody does not react with Horse.

Immunogen

Human thymocytes and T lymphocytes.

Host

Mouse

Clonality

Monoclonal

Clone ID

MEM-28

Isotype

IgG1

Conjugate

Alexa Fluor® 700

Excitation: 702nm, Emission: 723nm

Purification

This antibody is conjugated with Alexa Fluor® 700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 15mM Sodium Azide.

Storage

Store in the dark at 2-8°C. Do not freeze!

Synonyms

L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

Isotype Controls

Mouse IgG1 [MOPC-21] (Alexa Fluor® 700) (A18784)

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Flow Cytometry - Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (A18760)

Flow cytometric analysis of human peripheral whole blood stained with Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (4µl reagent / 100µl of peripheral whole blood).

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Flow Cytometry - Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (A18760)

Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood stained with Anti-CD45 Antibody [MEM-28] (Alexa Fluor® 700) (4µl reagent / 100µl of peripheral whole blood).