Mouse monoclonal [MEM-28] antibody to CD45 (Biotin).
Specificity
This antibody reacts with an extracellular epitope on all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Applications
Flow Cytometry, IP, WB, IHC-P, ICC
Dilutions
Flow Cytometry: 1 µg/ml
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Horse.
Immunogen
Human thymocytes and T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-28
Isotype
IgG1
Conjugate
Biotin
Purification
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentration
1 mg/ml
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store at 2-8°C. Do not freeze!
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Anti-CD45 Antibody [MEM-28] (Biotin) (A85893) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Jurkat, Raji, HeLa, and HEK293T cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-28 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD45 molecules were detected at ~180-250 kDa in Jurkat and Raji cell lines.
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