Rat monoclonal [YKIX753.22.2] antibody to CD45R (PE).
Specificity
This antibody recognises canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts,. It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl
Reactivity
Canine
Immunogen
Canine thymocytes.
Host
Rat
Clonality
Monoclonal
Clone ID
YKIX753.22.2
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Dog CD45R antibody, clone YKIX753.22.2 recognizes canine CD45R and was clustered at the First Canine Leucocyte Antigen Workshop (CLAW). It precipitates an antigen of 220 kDa from Con A Blasts, (Cobbold et al. 1994). It is expressed on subsets of leucocytes in canine blood of all outbred mongrels and beagles tested. Rat anti dog CD45R, clone YKIX753.22.2 binds to antigen immunoprecipitated by the Rat anti Dog CD45 antibody clone YKIX716.13 (Cobbold et al. 1994).
Figure A. Alexa Fluor 488 conjugated Rat anti Canine CD44 and Rat IgG2a (PE) - Isotype Control (A282640). Figure B. Alexa Fluor 488 conjugated Rat anti Canine CD44 and Anti-CD45R Antibody [YKIX753.22.2] (PE) (A282060). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Figure A. Anti-CD45R Antibody [YKIX753.22.2] (PE) (A282060) and Alexa Fluor 488 conjugated rat IgG1 isotype control. Figure B. Anti-CD45R Antibody [YKIX753.22.2] (PE) (A282060) and Alexa Fluor 488 conjugated rat anti Canine CD44. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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